Dear GFP guru's,
It seems that when the cell express GFP in the cytoplasm (mammalian
cells), it will leak from the cell even if it has been paraformaldehyzed
(10-30% of the total content, O/N, 4C). I suppose that this phenomenon
is not related to the degradation of GFP (ref. search GFP
paraformaldehyde in FLUORESCENT-PROTEIN LIST).
Could you refer me to a fixation protocol that could prevent this
leaking for 4 to 5 days ? =3D=3D total impermeabilization of the cell ?
Thank you for your help.
>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
Philippe-Alexandre Gilbert
Institut de recherche en biotechnologie
Conseil national de recherches Canada
6100 avenue Royalmount, Montr=E9al QC H4P 2R2
t=E9l: (514) 496-6252
fax: (514) 496-6785
Internet: aaa001 at agora.ulaval.ca
