Permeability and Paraformaldehyde

Philippe-Alexandre Gilbert aaa001 at AGORA.ULAVAL.CA
Fri May 29 09:04:26 EST 1998

Dear GFP guru's,

It seems that when the cell express GFP in the cytoplasm (mammalian
cells), it will leak from the cell even if it has been paraformaldehyzed
(10-30% of the total content, O/N, 4C). I suppose that this phenomenon
is not related to the degradation of GFP  (ref. search GFP
paraformaldehyde in FLUORESCENT-PROTEIN LIST).

Could you  refer me to a fixation protocol that could prevent this
leaking for 4 to 5 days ? =3D=3D total impermeabilization of the cell ?

Thank you for your help.

Philippe-Alexandre Gilbert

Institut de recherche en biotechnologie
Conseil national de recherches Canada
6100 avenue Royalmount, Montr=E9al QC H4P 2R2

t=E9l: (514) 496-6252
fax: (514) 496-6785

Internet: aaa001 at agora.ulaval.ca

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