I have tried the perplasmatic expression EGFP in E.coli.
The westerblots shows that the concentration of EGFP is at least 10
microgramms per 1ml, but I could detect hardly any fluorenscence signal.
Does anyone has some done perplasmatic expression EGFP in E.coli? or has
an idea what the problemo might be.
Thank for your help.
Protein Structure Research Labs
Monbijou Str. 2
e-mail: mpaschke at rz.charite.hu-berlin.de