To: fluorpro at net.bio.net
From: lapointr at post.queensu.ca (Renee Lapointe)
Subject: EGFP sensitivity augmentation by anti-GFP Abs
Date: 14 Oct 1998 12:43:11 -0700
Hello everyone,
I have two questions for the group.
1) Therefore we would like to know whether the use of a
GFP specific antibody could improve the signal. Has anyone tested that?
*Yes, I've tested Chemicon's chicken anti-GFP with good success. I've been able to use it at
*a 1:5000 dilution in immunohistochemistry, and at around 1:20,0000 dilution on western
*blots. This may be a better bet for you, given question #2 as well.
2) I guess this brings me to the second part of my message. CLONTECH, the
company which sells these vectors, also sells a polyclonal antibody against
GFP, but it's titer for immunofluorescence is 1/200, rendering the cost of
its use too high for us to use (we have numerous promoters and conditions
to test). Does anyone knows of a cheaper source of GFP-specific antibody
which would detect EGFP even in a denatured form (for westerns for example)?
Thank you for your help,
Renee Lapointe
Rene Lapointe
Department of Microbiology and Immunology
Queen's University, Kingston, Ontario, K7L 3N6
CANADA
Tel.: (613) 545-6702, FAX: (613) 545-6796
E-mail: lapointr at post.queensu.ca
