DENISE at cellbio.emory.edu wrote:
>> To: fluorpro at net.bio.net> From: lapointr at post.queensu.ca (Renee Lapointe)
> Subject: EGFP sensitivity augmentation by anti-GFP Abs
> Date: 14 Oct 1998 12:43:11 -0700
>> Hello everyone,
> I have two questions for the group.
>> 1) Therefore we would like to know whether the use of a
> GFP specific antibody could improve the signal. Has anyone tested that?
>> *Yes, I've tested Chemicon's chicken anti-GFP with good success. I've been able to use it at
> *a 1:5000 dilution in immunohistochemistry, and at around 1:20,0000 dilution on western
> *blots. This may be a better bet for you, given question #2 as well.
>> 2) I guess this brings me to the second part of my message. CLONTECH, the
> company which sells these vectors, also sells a polyclonal antibody against
> GFP, but it's titer for immunofluorescence is 1/200, rendering the cost of
> its use too high for us to use (we have numerous promoters and conditions
> to test). Does anyone knows of a cheaper source of GFP-specific antibody
> which would detect EGFP even in a denatured form (for westerns for example)?
>> Thank you for your help,
>> Renee Lapointe
>> Rene Lapointe
> Department of Microbiology and Immunology
> Queen's University, Kingston, Ontario, K7L 3N6
> CANADA
> Tel.: (613) 545-6702, FAX: (613) 545-6796
> E-mail: lapointr at post.queensu.ca
Affinity BioReagents, Inc. recently released a rabbit polyclonal
anti-GFP antibody (Cat. # PA1-980) which works nicely in Western blots
(ECL detection) at 1:2,000 and higher dilution. Please contact us for
details.
Sincerely,
Phil
