I use Chemicon's Chicken A'GFP (AB16901---$200 for 250ug). It works great. I've used it in
neuronal cell cultures and fibroblast cell cultures. The 250ug should take you a long way. I
get good results @ about 1:2500 dilution for immunohistochem. For westerns w/ECL I've
been able to use it at 1:20,000 and could probably go even more dilute. If you want my IH
protocol, just e-mail me.
To: fluorpro at net.bio.net
From: logg at hsc.usc.edu (Christopher Logg)
Subject: immunohistochem with commercial GFP Ab
Date: 1 Apr 1999 17:20:37 -0800
Reply-to: logg at hsc.usc.edu
Could anyone recommend a commercially available anti-GFP Ab that they
know works well for immunohistochemisry?
USC School of Medicine