I'm expressing a conjugate containing ECFP and EYFP from
'yellowcam-2' ( Miyawaki et al.,(1997)), and I have found that
the EYFP fluorescence intensity is considerably lower than reported
in the paper. This may be due to my particular construct, which
contains a different binding region to the original. However, I have
heard reports that EYFP intensity in original constructs varies
with pH and ion concentration. Can anybody support this? If so,
what conditions gain optimal EYFP intensity?
I would greatly appreciate any advice or comment from anyone using
these 'Cameleons', in ANY system.
Thank-you,
Emma Stavropoulos.
e:mail: Emma.Stavropoulos at ed.ac.uk
Emma Stavropoulos
Institute of Cell and Molecular Biology
University of Edinburgh
Tel (+44) (0)131 6505319
Fax (+44) (0)131 650 5392
Emma Stavropoulos
Institute of Cell and Molecular Biology
University of Edinburgh
Tel 0131 6505319
