I have got the same question as Cheryl Wellington.
Has anyone ever made a GFP fusion protein (or e.g. BFP) without any
detectable flourescent after expression in mammalian cells?
Michael
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Dr. Michael Kubbutat
Institute of Molecular Medicine
Tumor Biology Center
Breisacher Strasse 117
79106 Freiburg
Germany
Tel.: +49 (0) 761 206 1600
Fax.: +49 (0) 761 206 1705
e-mail: kubbutat at tumorbio.uni-freiburg.de