Brian D. Knutson wrote:
> I am still looking for a reproducible purification scheme for the
> wtGFP. I am doing a research project for an advanced undergrad biology
> class that involves the purification of this protein and haven't had any
> luck finding a definite easy to follow procedure. If anyone has
> information on where I could find a good purification scheme please
> contact me. I will be starting this project within the next 3-4 weeks.
Have you considered using his-tag wt-GFP ? Purification with
a Co or Ni column is very easy.