wtGFP purification

Peter Schellenberg schelli at u.washington.edu
Sun Apr 18 18:42:52 EST 1999

Brian D. Knutson wrote:

> I am still looking for a reproducible purification scheme for the
> wtGFP.  I am doing a research project for an advanced undergrad biology
> class that involves the purification of this protein and haven't had any
> luck finding a definite easy to follow procedure. If anyone has
> information on where I could find a good purification scheme please
> contact me.  I will be starting this project within the next 3-4 weeks.

  Have you considered using his-tag wt-GFP ? Purification with
a Co or Ni column is very easy.


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