> Has anyone gotten away with using ampicillin as a
> selective antibiotic for this vector
I'll be amazed if they have!
pEGFP-N1 does not have an ampicillin resistance gene, therefore, you will have
to use kanamycin.
-=Paul=-
""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
" Paul Kitts, Ph.D. NIH/NLM/NCBI "
" Email: kitts at ncbi.nlm.nih.gov Bldg 38A, 8N803 "
" Phone: 301 435-6091 8600 Rockville Pike "
" Fax: 301 480-9241 Bethesda, MD 20894, U.S.A. "
""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
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To: fluorpro at net.bio.net
From: Dr Lester Davids <lester at uctgsh1.uct.ac.za>
Subject: pEGFP-N1?
Date: Thu, 29 Apr 1999 17:16:39 +0200
I am using the pEGFP-N1 vector and really struggling to transform high
competent JM109's. Has anyone gotten away with using ampicillin as a
selective antibiotic for this vector or must one use kanamycin?
Also, has anyone had success with using more than 1-10ng DNA of this
vector in the transformation reaction?
Thanx
