IUBio

GFP work in vitro

Guy Tremblay guyt at istar.ca
Mon Aug 9 23:30:32 EST 1999


Weidong Xie wrote in message <37ADDB6B.B43644EE at stjude.org>...
>Hi, I am trying to fusion antibodies with GFP, Is this protein working
>in vitro, or any one else have tried this before??


It does fluoresce in vitro. The problem you might get is sensitivity since
GFP is not based on enzymatic activity. But since its in vitro, one just
leaves it on the film longer.





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