Hello!
I am working on a GFP fusion protein which localizes to the nucleus in
sea urchin embryos. I have tried a control experiment in which I
microinject only GFP - and this localizes throughout each cell
(cytoplasm, membrane, nucleus) but concentrates in the nucleus. Does
GFP alone normally accumulate in the nucleus? Can I be sure that the
localization I see with my fusion protein is entirely due to the
localization signal of protein and not GFP? Can anyone give me a
reference in which to find out if this is a common phenomenon? Finally,
does anyone know of a fluorescent molecule or dye that diffuses evenly
thoroughout a cell and does not get partitioned into the nucleus or
other subcellular structures?
Thank you so much,
Heather
