Hi There, is your protein soluble? if it is then the protein can be
washed out if the cells are not fixed first!! I've found that with GFP
alone, although membrane receptors tagged with GFP are fine following
methanol fixation.
Hope this helps
Andy
"m.hagedorn" wrote:
> Hi there
> I am doing colocalization of a GFP-tagged protein and using
> immunofluorescence.
> I found that after permeabilizing the cells with 0.1% tritonX100 in
> 1xPBS the GFP-signal disappears or weakens.
> Did anyone have the same observation or can help me?
> Thanks
