IUBio

GFP in yeast

AG Entian eschrich at em.uni-frankfurt.de
Tue Dec 14 11:04:38 EST 1999


Hi

I have localized a protein in S. cerevisiae by GFP-fusion. It seems to
be a microtubule associated protein. A quite interesting phenomenon is,
that if I incubate my strain for some days in the cold the localization
is much nicer. Has anyone seen such a phenomenon or could someone
explain this to me ?
Thanks
Dietmar




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