Hi Deb,
We have expressed the pDsRFPN1-1 version of RFP using the attached CMV promoter
in transgenic zebrafish and it has worked fine. We could see it in cells; a
little fuzy because it was just RFP alone and was not localized to any sites
within the cell and due to the 3D embryo. This is the human codon optimized
version which was suggested to be used in mammals. The wild type version was
suggested for use in nonmammals and we are in the process of generating
transgenic fish expressing RFP fused to our protein of interest. That's all
from Atlanta-hope it helps.
Henryt
Henry G. Tomasiewicz, Ph.D.
Emory University
Department of Cell Biology
1648 Pierce Dr.
Atlanta, GA 30322
Ph 404 727-6255
fax 404 727-6256
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