Dear Serge,
you can obtain the GFP-sg25 vector from Quantum Biotechnologies Inc.
Check out this site for further information:
http://www.qbi.com
They say that the increase in green fluorescence is 50-100x higher
when compared with wtGFP. EGFP is about 35fold brighter than wtGFP.
Felix
> Does anyone know if DNA coding for the GFP sg25 described by Stauber et
> al. in Biotechniques 24(3), 462 (1998) is available or can be purchased
> somewhere? Is the fluorescence stronger with this mutant compared to
> Clontech's EGFP?
> Thanks for any help,
> Serge
Felix Bestvater
Deutsches Krebsforschungszentrum
Abt. Biomedizinische Strukturforschung (A0600)
Im Neuenheimer Feld 280
D-69120 Heidelberg
TEL: (+49) 06221 - 42 34 32 (33)
Fax: (+49) 06221 - 42 34 59
Email: F.Bestvater at dkfz-heidelberg.de
