Dan-
If you got an NFAT/Luc reporter plasmid from Stefan Ho, an NFAT
re/minimal IL-2 promoter fragment drops out as ~200 bp BamHI-HindIII
fragment. It would be trivial to replace that into any eGFP expression
vector you have. The HindIII should be next to the protein reading
frame.
Beware, eGFP is very stable! Fold induction could be minimal at best if
you've got some leak in the cells.
--
T.J. Murphy, Ph.D. 404-727-2467
Assistant Professor 404-727-0365 (fax)
Department of Pharmacology tmurphy at pharm.emory.edu
Emory University School of Medicine
5031 O.W. Rollins Research Building
Atlanta, GA 30322
http://www.emory.edu/PHARMACOLOGY/MURPHY/
"If we're not driving paradigms, then we're out driving Titleists"
