Dear "Clontech" staff and all GFP fans,
Let us to present for you the two our recent publications devoted
to GFP expression under cardiac specific promoter wich once more
prove the great possibilities of living fluorescent reporters.
At least for us it has became the only possibility to identify
cardiac precursor cells during ES cells differentiation and
investigate their functional characteristics before onset of conractile
activity.
See: E.Kolossov et al.: Functional Characteristics of ES Cell-derived
Cardiac Precursor Cells Identified by Tissue-specific Expression of the
Green Fluorescen Protein. J.Cell.Biol.(1998),v.143, n7, 2045-2056 .
The second article shows the transgenic mice created with construct
used in above mentioned one with cardiac specific GFP expression on
early
embryonic stages. See: M.Fleischmann et al.: Cardiac specific expression
of the green fluorescent protein during early murine embryonic
development.
FEBS Letters (1998), 440, 370-376. This article with pictures of our
"green heart" embryos
is also available for free in PDF format by next www.address:
http://www.elsevier.nl:80/cgi-bin/cas/tree/store/febs/cas_free/browse/browse.cgi?year=1998&volume=440&issue=3
Please, if you are interesting in applying of GFP to cardiac
differentiation studies,
send us your remarks regarding of these articles as well as your
experience in this field.
