IUBio

dual expression and time lapse

tomoko watanabe tw118 at COLUMBIA.EDU
Sun Feb 7 12:03:16 EST 1999


Hi!

I'm interested in expressing two fluorescent proteins in mammalian cells
for time lapse experiment.  We already cloned GFP in and would like to
use YFP/BFP or any other fluorescent protein as the second color.  Are
there anyone who has done this, or any recommendation for what protein I
should use for the second one?

Also...any informaiton on how to detect two fluorescent protein would be
appreciated.

Thank you

Tomoko




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