IUBio

Dual Expression for Time Lapse

Tong Zhang tz12 at COLUMBIA.EDU
Sun Feb 7 14:43:34 EST 1999


Hi!

I'm interested in expressing two fluorescent proteins in mammalian cells

for time lapse experiment.  We already cloned GFP in and would like to
use YFP/BFP or any other fluorescent protein as the second color.  Are
there anyone who has done this, or any recommendation for what protein I

should use for the second one?

Also...any informaiton on how to detect two fluorescent protein would be

appreciated.

Thank you

Tomoko




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