You are right. I analyzed cells in PBS.
"'Mike' Michael J. Moser" wrote:
>> On Tue, 9 Feb 1999, Eugene Kandel wrote:
> > Before reading I substituted medium for PBS, but I do not know how
> > important it is.
>> Do you mean substituted PBS for medium? I figured GFP would be harder to
> see in the higher fluorescent background generated by medium.
>> Thanks much for the advice!
>> Mike Moser, Ph.D. Tel: 206-543-6585
> UW Department of Pathology FAX: 206-543-3967
> Box 357705 moser at u.washington.edu> Seattle, WA 98195 http://weber.u.washington.edu/~moser
Dr Eugene S. Kandel
The University of Illinois at Chicago
Department of molecular Genetics (m/c 669)
900 S. Ashland Ave, Rm 2106
Chicago IL, 60607