Detecting GFP expression driven by mammalian promoters

['Mike' Michael J. Moser]

On Tue, 9 Feb 1999, Eugene Kandel wrote:
...
> Before reading I substituted medium for PBS, but I do not know how
> important it is.
....

Eugene

Do you mean substituted PBS for medium?  I figured GFP would be harder to
see in the higher fluorescent background generated by medium.

Thanks much for the advice!

Mike

Mike Moser, Ph.D.                                     Tel: 206-543-6585
UW Department of Pathology                            FAX: 206-543-3967
Box 357705                                       moser at u.washington.edu
Seattle, WA  98195                 http://weber.u.washington.edu/~moser