GFP & Inverted Microscopy

Tony Hodge tph at mrc-lmb.cam.ac.uk
Fri Feb 12 04:59:23 EST 1999

Dr Robert Gay <r.gay at unsw.edu.au> wrote:

> Dear GFPers
> We're looking for a microscope system for the visualisation of
> EBFP/EGFP/EYFP directly in culture flasks.
> At the momment we can visualise cells expressing FPs when cultured
> directly on coverslips and viewed using an Olympus BH2 microscope with
> the Reflected Light Fluorescence attachment.  Does anyone know of a
> similar system set up for an inverted microscope which allows direct
> visualisation in the culture flasks ?
> Thanks in advance

My experience with culture flasks has been dissapointing.  The plastic
is thick enough to warrant using a LWD objective and the ones we have
are not efficient enough (small NA) for epifluoresecent illumination.
So I have stuck with culturing on coverslips and mounting these in a
chamber supplied by Molecular Probes for viewing on an inverted

I have also seen on the market disposable 3.5cm culture dishes with
coverslip quality glass bases (also in other sizes).  These however were
not very cost effective so I've not tried them out yet.


Tony P Hodge
tph@*nospam*mrc-lmb.cam.ac.uk  (remove *nospam* )

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