We are expressing an intracellular transporter protein tagged with EGFP in
a neuroblastome cell line. Although most cells express the fusion protein
in what appears to be the correct location, we also see nuclear labeling,
which should not be present at all. Because it is a minority of the
transfected cells that present this wrong localization, I was wondering
whether other people had seen a similar phenomenon. . I was wondering
whether that could happen because of clivage of the fusion protein and
diffusion of EGFP to the nucleus. Transfection of EGFP alone labels the
whole cell, including the nucleus.
Thanks for the help.
--
Marco
Dr. Marco Antonio M. Prado
Laboratório de Neurofarmacologia
Departamento de Farmacologia
Instituto de Ciências Biológicas
Universidade Federal de Minas Gerais
Av. Antonio Carlos, 6627
Belo Horizonte, MG, Brasil
31270-901
voice 55 31 499 2718
fax 55 31 499 2695
http://www.icb.ufmg.br/~neurofar
