EGFP Detection?

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Mon Feb 22 20:48:42 EST 1999

In article <36D1EA30.A68FC68 at unm.edu>, pkim at UNM.EDU ("Perry Kim, Ph.D.") wrote:

> Can anyone please tell me if EGFP can be detected in living mammalian
> cells.  In other words can I take a 10 cm dish containing EGFP
> expressing cells and visualize them unsing fluorescent microscopy?
> Thanks in advance.

Basically, yes.  It depends how strong your promoter is.
Also, see previous posts concerning choosing the best
culture medium to reduce fluorescence from that.
     Glow ahead and try it...
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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