FIaxtion of GFP

Derya is an idiot unutmaz at MCBI-34.MED.NYU.EDU
Wed Feb 24 13:14:27 EST 1999

I generated GFP knockin mice. I found GFP fluorescence disappears after
freeze-thaw, unless pretreated with 4% paraformaldehyde. However, such
fixation will destroy/mask the cell surface epitope. Therefore, I can
not do staining for cell surface markers.

Does anyone have any experience on this?


More information about the Fluorpro mailing list

Send comments to us at biosci-help [At] net.bio.net