Hi,
1. Can anyone get good result in CFP/YFP FRET experiment?
2. I tried to use XF88 CFP\YFP filter set from Omega filters to perform this
experiment, but i can see the YFP fluoresence using the exciter (440DF21)
and emitter 2 (535DF26)of XF88 fitler set , Why? Since the cells were
transfected with EYFP-C1 vector only.
3. Is it YFP can exciter effectively at 440 nm?
4. I am using ECFP-C1 and EYFP-C1 for this experiment, are they the suitable
partner?
5. Or the filter are not suitable for it?
Thank you for any reply
Hoi Yeung, Li
Department of Biochemistry
CUHK
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