Detection of BrdU incorporation and GFP fluoreszence

Christian Zuppinger zuppinge at cell.biol.ethz.ch
Tue Jun 15 00:36:20 EST 1999


I am looking urgently for a protocol allowing the simultaneous detection
of GFP and BrdU incorporation.
For BrdU incorporation studies, until now I used the kit form Boehringer
Mannheim (now Roche diagnostics).
The problem with this kit is, that cells are fixed in an ethanol-Glycine
buffer (pH 2), which destroys GFP fluorescence. Does anybody have an
idea, how to solve this problem?
PFA fixation and permeabilisation with 0.1% Triton does not work.



Josef P. Magyar, Ph.D.
ETH Hoenggerberg
Institut fuer Zellbiologie, HPM F27
CH-8093 Zurich

Tel: + 441 1 633 33 54
Fax: + 441 1 633 10 69
Email: magyar at cell.biol.ethz.ch

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