Clontech's pX-IRES-EGFP

Dr. Robert Gay r.gay at unsw.edu.au
Tue Jun 15 19:43:14 EST 1999

Dear GFPers

Has anyone successfully got reasonable expression levels of EGFP from the
attenuated IRES vector p-IRES-EGFP (or EYFP for that matter) ?

If so I'd be interested to hear which cell line you're using, whether
expression is transient or stable and what transfection techinique you're

I seem to be having a hard time in CHO cells at the momment.

Any help will be greatly appreciated.



Dr Robert Gay

Post Doctoral Research Fellow
CRC for Biopharmaceutical Research
Department of Biotechnology
University of New South Wales
Sydney, NSW 2052, Australia

Tel : +61(0)2-9385-1892
Fax : +61(0)2-9313-6710

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