"Dr. Robert Gay" <r.gay at unsw.edu.au> wrote:
> Has anyone successfully got reasonable expression levels of EGFP from the
> attenuated IRES vector p-IRES-EGFP (or EYFP for that matter) ?
Well, it depends of your definition of "reasonable".
In my hands the expression of EYFP is about 200 times less that the first
protein, but it still
detectable in live 10T1/2 cells in PBS with omega X104 filter set.
So, I'm hardly wayting for new less attenuated pIRES construct.