Christer Ericsson <ericsson at acsu.buffalo.edu> wrote:
> What I really would need for a marker would be a good "prototypical"
> GFP-labeled glycosylated integral membrane protein, which sorts to the
> plasma membrane efficiently enough to serve as a marker.
Stable cell lines with about any GPCR work just fine :-) Personally, I
use beta2-adrenergic receptor which has the disadvantage that it will
internalize upon addition of agonist, but there are other GFP-tagged
GPCRs out there which might be more trafficking-resistant.
OTOH, I remember reading an article in Biochemical Journal some time
ago where the plasma membrane was counterstained by trypan blue
fluorescence. Is there any particular reason that you want to use a
PS: Next time you want to post something in multiple newsgroups, please
use crossposting instead of multiposting. Thank you.
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