Liao Cheng <chliao at online.sh.cn> wrote:
> Would I fuse my protein into the N-terminal or C-terminal of
> GFP? Which will be better?
You have to try it for yourself. Some proteins will tolerate N-terminal
fusions better than C-terminal ones, others vice versa and still others
won't tolerate either.
As for making the fusion, it's just ordinary molecular biology - no
special protocols needed.
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
/* "Science is the game we play with God to find out what His rules are." */