to my knowledge, only some (i.e. less than 10) AAs at the c
term may be deleted, if you don't want to abolish fluorescence.
I don't remember the reference though.
You might be lucky at clontech's website (www.clontech.com) they have
a comprehensive manual on gfp for download.
Another good source is a special issue of Trends in Cell Biology
1996, 7 or 8 on GFP
In general, a medline search for the basic papers by Tsien might be
> From: zheng at brookes.ac.uk (H_Zheng)
> Subject: fluorescence of GFP in tolerance in its aa deletion?
> Date: 17 Nov 1999 05:51:01 -0800
> Organization: BMS
> Reply-to: zheng at brookes.ac.uk> To: fluorpro at hgmp.mrc.ac.uk
> Dear friends,
>> I am apppreciated if any one can point me any references, the newest
> data on the fluorescence of GFP in tolerance of its aa deletion at
> both N- C- termini on the optimal condition.
>> Thank you
>> Hugo Zheng
usual disclaimers apply
Dr. Wolfgang Schechinger
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de
*unsolicited mail is *NOT* appreciated