absorption coefficient

[Platini Kwok]

Hi Paul,

I should say the absorption coefficient of gfp is changing with conditions
such as pH, effects of other protein and etc. It is very difficult to make
sure the matrix effects in each experiment the same, therefore, it is no use
if you even know the absorption coefficient. That's why manufacturer don't
include the factor in the kit. In short, it seems to me that it is easy to
determine the concentration of gfp by Beer's law.

Platini

Paul Foster ¼¶¼g©ó¤å³¹ <382B1121.AD59EB4A at msg.ucsf.edu>...
>What is the (molar) absorption coefficient of GFP (and its variants?)
>
>Thanks
>