Platini,
it seems to me that the absorption coefficient is necessary for the
application of Beer's law. wich is E = (epsilon)*c*d where (epsilon
is the absorption coefficient, c is concentration and d is thickness
of the cuvette, E is absorption, sometimes (in Germany) also referred
as extinction). Given similar or identical matrices, it should be
possible to determine ratios of GFP in different samples assuming
identical pH and temperature in every sample.
Wolfgang
> From: "Platini Kwok" <b965772 at cuhk.edu.hk>
> Subject: Re: absorption coefficient
> Date: Fri, 19 Nov 1999 09:59:25 +0800
> Organization: The Chinese University of Hong Kong
> To: fluorpro at hgmp.mrc.ac.uk
> Hi Paul,
>> I should say the absorption coefficient of gfp is changing with
> conditions such as pH, effects of other protein and etc. It is very
> difficult to make sure the matrix effects in each experiment the
> same, therefore, it is no use if you even know the absorption
> coefficient. That's why manufacturer don't include the factor in the
> kit. In short, it seems to me that it is easy to determine the
> concentration of gfp by Beer's law.
>> Platini
>> Paul Foster +Â+g®¾ñÕ¦¦ <382B1121.AD59EB4A at msg.ucsf.edu>...
> >What is the (molar) absorption coefficient of GFP (and its
> >variants?)
> >
> >Thanks
> >
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Dr. Wolfgang Schechinger
Pathobiochemistry Dept.
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de
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