Dear All
Has anyone done a lot of colocalisation studies using GFP tagged
transfected constructs and a red-tagged antibody? If so has anyone ever had
the problem of the GFP green signal losing its fluorescence after
permeabilisation? Does anyone know any handy hints to get around this at
all?
Thanks for your help
Kate
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Kathryn Sunn BSc (Hons)
Postgraduate Student
Bone and Mineral Research Program
Garvan Institute of Medical Research
384 Victoria St, Darlinghurst
St Vincent's Hospital Tel: (02) 9295 8260
Sydney NSW 2010 Fax: (02) 9295 8241
Australia email: k.sunn at garvan.unsw.edu.au
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