Wolfgang's reply raises an interesting and very important question which
could be discussed here: Which influence has FRET-induced reduction of
donor fluorescence on the results of double labeling immunofluorescence
microscopy in general? Could it be, that other dye systems as well (such as
FITC/TMR etc.) often don't show us the real colocalizers - due to quenching?
Has anybody ever systematically investigated this problem - i.e. by
comparing the results of green/red vs. blue/red dye pairs?
Jacques
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Jacques Paysan, PhD
Universität Hohenheim
Institut für Physiologie (230)
Garbenstrasse 30
70593 Stuttgart
Tel. 0711-4592267
Fax. 0711-4593726
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