Hello,
you could try engineering in a protease recognition sequence between the GFP
and your protein:
for example:
Enterokinase site cuts at ^
DDDDK^D
(I have not tried this - so I don't know if it will work!)
Good luck
yue jiang wrote in message <380A354A.889E069C at iastate.edu>...
>Hi, all,
>>I plan to make a GFP fusion protein.
>>I want to know if there is any methods to quench or specifically degrade
>GFP but keep the other part of my fusion protein intacted.
>>Any suggestion are very appreciated.
>>Qi
>e-mail: qizhang at iastate.edu>
