IUBio

Western and IP

Linda Mills linda at playfair.utoronto.ca
Wed Sep 22 10:17:07 EST 1999


We have used Molecular Probes anti-GFP with very good results,  note their newer
one which is supposedly for IP & westerns  was not as good as the older one. It
is expensive though, any chaeper ones out there??

Cornelius Krasel wrote:

> Sam Straight <sstraigh at umich.edu> wrote:
> > Does anyone know good GFP antibody ? We have tried using Clontech " the
> > living color " in western blot. It did not work. Has anyone successfully
> > using the antibody  for western blot and IP?
>
> I have used the polyclonal antiserum from Clontech successfully in
> Western blots.
>
> --Cornelius.
>
> --
> /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
> /* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
> /* "Science is the game we play with God to find out what His rules are."  */

--

Dr. Linda  R. Mills Ph.D.
Staff Scientist, Toronto Western Hospital Research Institute &
Associate Professor,  Dept. Physiology
University of Toronto
University Health Network
11-430 TWH
399 Bathurst St.
Toronto On
M5T 2S8





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