GFP-fusion toxicity

hedong at rci.rutgers.edu hedong at rci.rutgers.edu
Wed Apr 12 11:15:55 EST 2000

I fused a putative dominant-negative form of a transcription factor with
GFP to study its role in cell proliferation. I transciently transfected
this construct along with GFP alone into C6 glioma cell line. I am
seeing the effect, but cells transfected with GFP fusion protein, which
have green nucleus are dying. Does anybody have any information about
toxicicty of GFP-fusion protein, especially in the nucleus?

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