Hi,
I wanted to measure protein-protein interactions using Clontech´s DsRed in
FRET- or BRET-assays, but havn´t see much effects so far. So my question is:
Had anyone seen significant FRET between GFP and the Red-FP or BRET between
Renilla luciferase and RFP ?
I didn´t see much effects even with a GFP-RFP fusion protein, but I can not
rule out that this might be a specific problem of my construct.
So if anyone has positive or negativ proof for FRET or BRET using RFP,
please send a comment.
Sincerly,
Holger Fehr
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Dr. Holger Fehr
Inst. Physiological Chemistry II
University of Wuerzburg
Germany
fehr at biozentrum.uni-wuerzburg.de
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