Media for growing Ecoli/GFPuv

Paul Monaghan p.monaghan at ic.ac.uk
Wed Feb 16 14:46:25 EST 2000

Can someone help?

I have transformed an E. coli with pGFPuv vector from Clontech. I am trying
to monitor the growth of this bacterium in real-time. One of the problems I
am having is the background fluorescence of my media. Currently, I am using
LB-broth which fluoresces like crazy.  I am not so sure what it is in the
media that fluoresces, I imagine it could be several things. Does anyone
have any suggestions on an alternative media that can be made easily or
better still bought pre-made that has a lower or no background fluorescence?
Any suggestions would be most appreciated



     e-mail p.monaghan at ic.ac.uk
     Tel:  00 44 (0)171 59 45749
     Fax:  00 44 (0)171 59 45833

 Zeneca/Smithkline Beecham Centre for Analytical science
 Dept. of Chemistry
 Imperial College of Science, Technology and Medicine
 Exhibition road, South Kensington, London, SW7 2AY


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