We have tried but couldn't detect DsRed by flow cytometry.
DsRed probably isn't excited enough at 488 nm.
Gregers J. Gram
Laboratory for Infectious Diseases 144
H:S Hvidovre Hospital, Denmark
----- Oprindelig meddelelse -----
Fra: "MILLINGTON, Michelle [JJRAU]" <MMILLING at MEDAU.JNJ.com>
Til: <fluorpro at hgmp.mrc.ac.uk>
Sendt: 17. februar 2000 00:46
Emne: dsRFP detection
>> Just a query about Clontech's dsRFP......
>> We would like to use it in combination with EYFP and are wondering if anyone
> has had any experience with it. Has anyone been able to detect it by flow
> cytometry using the conventional filters for PE (FL-2)???
>> We have tried EBFP but are unable to detect by flow due to low fluorescence
> and photobleaching. Is dsRFP brighter and more resistant to photobleaching
> than EBFP?
>> Thanks for any input,
>>> Michelle Millington
> School of Physiology & Pharmacology
> University of New South Wales
> Sydney NSW 2052