Hi Paul
You probably have problems with the riboflavin in the media, which produce
much of the autofluorescence in the media I have used (DMEM). In an article
from Zylka & Schnapp in Biotechniques vol. 21, nr. 2 (1996), they use
either riboflavin-free media or UV-fotobleaches the media before adding it
to the cells.
Hope the help is any good
Sincerly yours Hans
Hans P.M.M.Lauritzen, B.Sc., M.Sc., Phd-student.
Copenhagen Muscle Research Center, The Panum Institute 12.4
Blegdamsvej 3
Copenhagen, Denmark
E-mail: H.P.Lauritz at mfi.ku.dk
Phone: +45 35327375 or +45 35327436, Private:+45 23453335
Fax: +45 35327420
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