Dear all,
Of course GFP is a tool developped for living organisms studies, but
I was still wondering if ever it could resist to some kind of
treatment for fixation.
Indeed I have GFP tagged bacteria, and I look their localisation with
confocal microscope but I d like to couple this with some more
classical fluorescence microscopy which needs making thin cuts of
fixed tissues. So is there a method that would allow me to fix
without destroying GFP.
thanks in advance
tristan
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Tristan Boureau
Biocenter Helsinki, Department of Biosciences
Division of General Microbiology, PO Box 56
FIN-00014 University of Helsinki, FINLAND
E-mail tristan.boureau at helsinki.fi
tel: (00 358) (09)708 59 655
(00 358) (0)50 329 58 07
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