DNA concentration using GFP?

Andrew Louka andrew.louka at embnet.uio.no
Fri Jan 7 10:24:30 EST 2000

Hello everyone,

I've heard that it's possible to use GFP and a 96-well plate 
fluorescence detector (e.g. Victor) to determine the concentration of 
purified genomic DNA.  I heard that this method was used when one has 
very little sample i.e. too little to put onto a spectrophotometer.

Can anyone tell me how this is done?
How accurate is it compared to spec. analysis?

Many thanks,


Institute of Immunology
University of Oslo, Norway
WWW: http://www.sequenceanalysis.com/

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