Your are confused,,,,, there is a fluorescent dye called Picogreen. you
add the sample and the dye and you need a fluorimeter. Check out
biorad, perkin elmer etc for equipment and reagents needed. GFP has
nothing to do with dna conce measurements.
Andrew Louka wrote:
> Hello everyone,
>> I've heard that it's possible to use GFP and a 96-well plate
> fluorescence detector (e.g. Victor) to determine the concentration of
> purified genomic DNA. I heard that this method was used when one has
> very little sample i.e. too little to put onto a spectrophotometer.
>> Can anyone tell me how this is done?
> How accurate is it compared to spec. analysis?
>> Many thanks,
>> Andrew
>> --
> Institute of Immunology
> University of Oslo, Norway
> WWW: http://www.sequenceanalysis.com/> ---
