Kathryn Sunn <k.sunn at garvan.unsw.edu.au> wrote:
> I was wondering if anyone has used the Clontech GFP polyclonal antibody and
> achieved a clean western blot?
Yes. We used the dilutions as suggested by clontech. As secondary, we
used a peroxidase-coupled anti-rabbit from Dianova (I think they re-sell
Jackson research antibodies) in a dilution of 1:3000. Development was
with ECL.
--Cornelius.
--
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
/* "Science is the game we play with God to find out what His rules are." */
