DsRed expression in E. coli

Isabelle Sagot isabelle.sagot at urz.uni-heidelberg.de
Mon Jun 19 09:54:50 EST 2000


Does anybody have been successfull in overexpressing DsRed in E. coli ?
If yes, what were the expression conditions ? (how many time induction,
temperature of induction, E coli strain....).
I have also problems for cloning the DsRed coding sequence (from the
clontech plasmid pDsRed-N1).
After cloning in my vector, the restriction map is OK but there is no
fluorescence (Microscope or spectrofluorimeter checking).
Any help is welcome.

Thank you very much


Dr. Isabelle Sagot
Biochemie-Zentrum Heidelberg
AG Hurt, 4. OG
Im Neuenheimer Feld 328
D-69120 Heidelberg
Tel: +49-(0)6221-54-4176
Fax: +49-(0)6221-54-4369
Email: hn7 at ix.urz.uni-heidelberg.de


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