In article <4.2.0.58.20001108134756.00a86860 at mail.sci.univr.it>,
portaluppi at sci.univr.it (Paolo Portaluppi) wrote:
> Hello.
> I'm planning to express DsRed and EYFP in Arabidopsis thaliana
> protoplast.
> I use currently the fluorescent proteins available at AIMS that, I know,
> were optimized for plant expression. Does anybody know if DsRed and EYFP
> are expressed without problems in plant systems?
> Thanks
>>> ---
We've tried to express some ER markers fused to DsRED (transiently) in
onion cells. We got cells that expressed the DsRED fusions but all we
could see were aggregates (inclusion bodies). I haven't tried any other
markers so far. Actually I have given up on the DsRED. EYFP expresses
fine both in transient systems as well as in the plant. However, some
people in the lab complain that the YFP is also more likely to form
aggregates in lines where the protein is expressed at high levels. I
haven't had much problems sofar but if you are making stable transgenics
it shouldn't be a problem since you will get a range of expression
levels anyway.
Good luck
G-J
