Does DsRed and EYFP work in plant?

Bao, Yiming ybao at noble.org
Thu Nov 9 12:24:59 EST 2000

I fused the DsRed to a nuclei localization protein.  In tobacco cells, the
transiently expressed fusion protein also forms huge aggregates in addition
to its normal nuclei localization.

Yiming Bao, Ph.D.
Noble Foundation
P.O. Box 2180
Ardmore, OK 73402

Tel: (580)221-7363
Fax: (580)221-7380
Email: ybao at noble.org

> -----Original Message-----
> From: Gert-Jan de Boer [mailto:geejay at andrew2.stanford.edu]
> Sent: Wednesday, November 08, 2000 9:50 PM
> To: fluorpro at hgmp.mrc.ac.uk
> Subject: Re: Does DsRed and EYFP work in plant?
> In article < at mail.sci.univr.it>, 
> portaluppi at sci.univr.it (Paolo Portaluppi) wrote:
> > Hello.
> > I'm planning to express DsRed and EYFP in Arabidopsis thaliana 
> > protoplast. 
> > I use currently the fluorescent proteins available at AIMS 
> that, I know, 
> > were optimized for plant expression. Does anybody know if  
> DsRed and EYFP 
> > are expressed without problems in plant systems?
> > Thanks
> > 
> > 
> > ---
> We've tried to express some ER markers fused to DsRED 
> (transiently) in 
> onion cells. We got cells that expressed the DsRED fusions but all we 
> could see were aggregates (inclusion bodies). I haven't tried 
> any other 
> markers so far. Actually I have given up on the DsRED. EYFP expresses 
> fine both in transient systems as well as in the plant. However, some 
> people in the lab complain that the YFP is also more likely to form 
> aggregates in lines where the protein is expressed at high levels. I 
> haven't had much problems sofar but if you are making stable 
> transgenics 
> it shouldn't be a problem since you will get a range of expression 
> levels anyway.
> Good luck
> G-J


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