I fused the DsRed to a nuclei localization protein. In tobacco cells, the
transiently expressed fusion protein also forms huge aggregates in addition
to its normal nuclei localization.
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Yiming Bao, Ph.D.
Noble Foundation
P.O. Box 2180
Ardmore, OK 73402
Tel: (580)221-7363
Fax: (580)221-7380
Email: ybao at noble.org
> -----Original Message-----
> From: Gert-Jan de Boer [mailto:geejay at andrew2.stanford.edu]
> Sent: Wednesday, November 08, 2000 9:50 PM
> To: fluorpro at hgmp.mrc.ac.uk> Subject: Re: Does DsRed and EYFP work in plant?
>>> In article <4.2.0.58.20001108134756.00a86860 at mail.sci.univr.it>,
>portaluppi at sci.univr.it (Paolo Portaluppi) wrote:
>> > Hello.
> > I'm planning to express DsRed and EYFP in Arabidopsis thaliana
> > protoplast.
> > I use currently the fluorescent proteins available at AIMS
> that, I know,
> > were optimized for plant expression. Does anybody know if
> DsRed and EYFP
> > are expressed without problems in plant systems?
> > Thanks
> >
> >
> > ---
>> We've tried to express some ER markers fused to DsRED
> (transiently) in
> onion cells. We got cells that expressed the DsRED fusions but all we
> could see were aggregates (inclusion bodies). I haven't tried
> any other
> markers so far. Actually I have given up on the DsRED. EYFP expresses
> fine both in transient systems as well as in the plant. However, some
> people in the lab complain that the YFP is also more likely to form
> aggregates in lines where the protein is expressed at high levels. I
> haven't had much problems sofar but if you are making stable
> transgenics
> it shouldn't be a problem since you will get a range of expression
> levels anyway.
> Good luck
> G-J
>>
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